ACTOne - An easily scaleable cAMP biosensor HTS platform for Gs and Gi GPCR drug discovery and deorphanization.

ACTOne is a highly-sensitive and cost effective tool for screening G-protein coupled receptors (GPCRs) that signal through Gs or Gi G-proteins. Using a natural biosensor approach, this technology has many advantages over biochemical or other live cell technologies.

ACTOne offers:

• Measurement of physiological levels of cAMP in living cells providing access to Gs and Gi receptors
• Excellent signal to noise ratio and reproducibility for better data confidence
• High sensitivity with ability to see cAMP changes in single cells
• Stable signal allowing kinetic or endpoint assay formats
• Screening of agonists and antagonists (or allosteric modulators) saving time and reagents
• Allows experimentation with endogenous receptors
• Cost-effectiveness
• Powerful deorphanizing tool to help study novel receptors
• Ability to work at room temperature making it more suitable to high-throughput screening
• Familiar protocols and equipment thereby simplifying validation and shortening integration time
• Ability to run as a homogeneous assay using membrane potential dyes
• Compatibility with single-cell imaging platforms for high-content analysis and multiplexing.

For additional information on ACTOne's features and benefits, click here.