BD™ CARV II is suitable for both live cell and fixed cell confocal imaging using a wide variety of fluorophores.

3D Reconstruction
Automation of internal multi-position excitation (8), dichroic (5) and emission (8) filter wheels allows multi-channel 3D and 4D images to be obtained quickly without compromising resolution. Using any of the recommended software packages, 3D and 4D reconstructions can be performed.

Sea Urchin Egg (Tubulin), Friday Harbor, UW George von Dassow, Ph.D.

Co-localization Measurements
Co-localization measurements can be performed on overlaid images using any of the recommended imaging software packages.

Time Laspe
Using any recommended imaging software and camera, time lapse imaging of cells or organisms can be performed for long periods of time without significant bleaching or phototoxicity. Depending on the speed of the physiology being measured, with the right camera and z-stepper combination, time lapse at a single plain or at multiple plains (4D) can be imaged.

Cell division C.Elegans (GFP-Histone, GFP Tubulin)

See our image gallery for more examples

High Speed Calcium Imaging
BD™ CARV II, in combination with intensified cameras and cameras with on chip amplification, can be used to image fast fluorescence changes at rates ranging from 50-100 frames per second.

50 frames per second using Photometrics Cascade 512B CCD Calcium sparks in Cardiac muscle

See our image gallery for more examples

Fluorescence Recovery After Photobleaching (FRAP)
FRAP is based on the principal of observing the rate of recovery of fluorescence due to the movement of a fluorescent marker into an area of the membrane which contains this same marker but which has been rendered non-fluorescent via an intense photobleaching pulse of laser light. The two-dimensional diffusion coefficient of the fluorophore is related to both its rate and extent of recovery. FRAP has proved to be a popular means to assess the structure of artificial and biological membranes.

A FRAP experiment and recovery kinetics using IP Lab (Scanalytics Inc.) software.

Dual Emission Imaging (FRET) CARV in combination with the Dual View™ adapter from Optical Insights allows you to perform: CFP/YFP and GFP/RFP Fluorescence Resonance Energy Transfer (FRET) Calcium imaging using dyes such as Fluo3 and Fura Red or dual emission Indo-1 imaging. Simultaneous FITC/TEX Red or FITC/ Rhodamine Imaging
Dual View™ and a CCD camera coupled to the CARV phototube.