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BD Pathway™ | Applications
Applications currently being run on the BD Pathway include:
- Apoptosis
- JC1
- YoPro
- Annexin V
- Nuclear fragmentation (DAPI or Hoechst)
- Cytotoxicity
- Live/Dead
- Ethidium homodimer/calcein AM
- Propidium iodide/fluorescein diacetate
- Mitochondrial Dysfunction
| Figure 1. Measurement of mitochondrial potential changes in M21 cells using TMRE.
Dose response curve of valinomycin activity measured by TMRE and images using the Pathway HT. |
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- NFkB Activation
- Translocation into nucleus
- Ion Channels and Ratioimaging
- Ca++ (Fluo-4, Fura-2)
- Na+ (SBFI)
- Ca++/Na+
| Figure 2. Differential cellular responses in calcium in VR-1 transfected NIH-3T3 cells.
Fura-2 calcium traces of individual cells indicate heterogeneous cellular responses to stimuli.
Cells courtesy Dr. Mike Iadorola, NIH.
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- Whole organism/tissue imaging
| Figure 3.
High resolution x,y,z positioning system allows precision capture of multiple images allowing imaging of larger specimens at very high resolution.
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| Figure 4. Confocal image of mitochondrial potential disruption in HEP-G2 cell line following addition of compound.
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