Powerful cell-imaging software for complete flexibility for drug screening and assay development, capture and analyze images and interpret data.

High content analysis using automated image-based technologies is one of the fastest growing areas in drug discovery. There is no limitation to the amount of biological information that can be found using the power of imaging. With the BD Pathway™, both AttoVision® software and Pattern Recognition: Image Interpretation Technology help the user extract and decipher this biological information.

The Pathway system is driven using the powerful AttoVision software.

• Pathway AttoVision Software

AttoVision Software

Designed to provide researchers with ultimate flexibility in designing and implementing cell based assays, the BD Pathway™ software integrates a novel data classification capability. After imaging at high resolution, cells are automatically identified and fluorescence intensity is monitored at the cellular or subcellular level. Using selective criteria, each cell can be automatically classified, during the experimental run, by a number of cell specific features (fluorescence intensity, rate of change, redistribution, etc.). Once classified, cells can be counted and traces color coded. Additionally, wells can be color-coded based on criteria selected by the user, enabling whole plate maps to identify particular responses of interest. The software also incorporates sophisticated 3D rendering capabilities allowing the system to be used as an automated confocal imaging workstation.

AttoVision Software offers:

• Pre-set assay protocols (view Applications)
• Open architecture which allows the user to create novel protocols
• Integrated image capture, analysis images and data interpretation

Novel Boolean data analysis quickly classifies cells into different functional categories.

• On-the-fly data presentation and analysis -using novel Boolean classification
• Endpoint, kinetic and time-course analysis (insert image: ROImask.bmp)
• Fully automated capabilities: autofocus, autosegmentation, autoanalysis
• Ability to switch between automated and manual mode for assay development
• Powerful kinetic interpretation algorithms
• Confocal Z-sectioning and 3D reconstruction capabilities

Powerful three-dimensional rendering capabilities.

Plate maps of drug discovery additions are easily imported and carried through the entire data set.

For more information on BD Pathway and AttoVision software, click here.

Pattern Recognition: Image Interpretation Technology

The Cytoprint program utilizes Atto's proprietary pattern recognition technology to automate image-based drug screening.

The Cytoprint technology allows:

• Faster algorithm development for assay development
• Ability to run more sophisticated assays which can be easily interpreted
• Creation of "fingerprints" of images that are searchable in a database
• Large number of biological events can be measured in a single experiment
• Identification of unknown or unanticipated responses
• Ability to identify earlier responses

For more information about becoming a beta test site or learn more about our current collaborative partnership program for applying Cytoprint technology to novel assay application and database solution contact Atto's Business Development group at Atto_Inquiry@bd.com or call 301-340-7320.