Imaging Platform

• Proprietary, fully integrated optical design
• Motionless stage handles 96 or 384 well plates, and microscope slides
• X,Y resolution: 0.05 µm (mechanical)
• Z resolution: 0.1 µm (mechanical)
• 5 images per second speed
• Olympus 20X/0.75na UApo/340 0.17 mm working distance objective
• 4X, 10X, 40X, 60X objectives also available.
• Integrated temperature and CO2 control

Confocal Unit

• Disk sampling rate: 200 fps (actual image capture rate depends upon camera speed)
• Operation mode: Confocal, widefield, brightfield
• Pinhole configuration: Nipkow spinning disk
• Pinhole size: 70 µm

Laser-Free Illumination

• Excitation: 340 nm to near-IR
• Light source: Dual HBO 100W mercury arc lamp units (xenon lamps available)
• White-light LED for transmission microscopy (with optional liquid handling head)

Filter configuration (user specified)

• Excitation: 16 filter positions/5 dichroic mirror positions
• Emission: 8 filter positions/5 dichroic mirror positions
• Independent dichroic mirror selection

Detection/Observation

• Internal high resolution Hamamatsu ORCA ER CCD camera
• Binocular eyepiece for direct sample viewing (optional)

Liquid Handling

• Motorized head with single-position syringe (2 - 100 µl nominal capacity)
• Mixing capability (tituration)

Software

• Automated focus and image acquisition
• Automated region of interest (ROI) identification
• Interactive data and image integration
• Concurrent mulitple ratiometric dye kinetics (patented)
• Z sectioning and 3D rendering
• Novel heirarchical data classification algorithm

Computer (minimum specification)

• Pentium IV, 1.8 GHz running Windows 2000 including DVD burner

Physical Dimensions (inches/centimeters)

• Width: 43/109
• Height: 66/168
• Depth: 36/91 (including keyboard shelf)